The manual for my AquaHot, model #AHE-100-02S, says on page 8, "Antifreeze Type Used..........Ethylene Glycol." On page 6 the manual says, "The basis for the Aqua-Hot heating system’s functionality is the antifreeze and water heating solution, which is comprised of water that is distilled, de-ionized, or soft, as well as antifreeze." A quick look through the manual did not reveal the antifreeze to water mixture ratio, but I assume it's supposed to be 50%-50%.